Human TNF alpha Recombinant Rabbit Monoclonal Antibody [PS00-40]
cat.: HA721265
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Cap)
Clonality: Monoclonal
Clone number: PS00-40
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Recombinant protein within full length protein.
Positive control: Recombinant TNF-alpha protein.
Subcellular location: Cell membrane, Secreted.
Recommended Dilutions:
  ELISA(Cap)

Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-07] to TNF alpha (Detector) (HA721266).
Uniprot #: SwissProt: P01375 Human
Alternative names: APC1 APC1 protein Cachectin DIF Differentiation inducing factor Macrophage cytotoxic factor Tnf TNF superfamily member 2 TNF superfamily, member 2 TNF, macrophage derived TNF, monocyte derived TNF-a TNF-alpha TNFA TNFA_HUMAN TNFSF2 Tumor necrosis factor (TNF superfamily member 2) Tumor necrosis factor alpha Tumor necrosis factor Tumor necrosis factor ligand superfamily member 2 Tumor Necrosis Factor, Membrane Form Tumor necrosis factor, soluble form
Images
HA721265_1.jpg Fig1: The binding activity of TNF-alpha (HA721265) with recombinant TNF-alpha protein.
Immobilized recombinant TNF-alpha protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721265) for 45min at 37℃.
HA721265_2.png Fig2: Standard curve of TNF-alpha matched pair antibodies: Sandwich ELISA analysis of TNF-alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721265 [PS00-40] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-alpha protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS01-07] (Biotin, 1:4,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, recombinant TNF-alpha protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.