Pyruvate Dehydrogenase E2 Recombinant Rabbit Monoclonal Antibody [JE34-38]
cat.: HA721267
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE34-38
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 69 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Pyruvate Dehydrogenase E2 aa 574-624/647.
Positive control: HL-60 cell lysate, Mouse heart tissue lysate, SK-Br-3 cell lysate, K562 cell lysate, Daudi cell lysate, human testis tissue, mouse cerebellum tissue, rat brain tissue.
Subcellular location: Mitochondrion matrix.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:2,000
1:200
Uniprot #: SwissProt: P10515 Human | Q8BMF4 Mouse | P08461 Rat
Alternative names: 70 kDa mitochondrial autoantigen of primary biliary cirrhosis Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex Dihydrolipoamide S Acetyltransferase Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial DLAT DLTA E2 E2 component of pyruvate dehydrogenase complex M2 antigen complex 70 kDa subunit mitochondrial ODP2_HUMAN PBC PDC E2 PDC-E2 PDCE2 Pyruvate dehydrogenase complex component E2 Pyruvate dehydrogenase complex E2 subunit
Images
HA721267_1.jpg Fig1: Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution.

Lane 1: HL-60 cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721267_2.jpg Fig2: Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/500 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: K562 cell lysate
Lane 3: Daudi cell lysate
Lane 4: HL-60 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721267_3.jpg Fig3: Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Pyruvate Dehydrogenase E2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 69 kDa
Observed band size: 69 kDa

Exposure time: 4 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721267_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721267_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721267_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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