Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE34-38 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 69 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human Pyruvate Dehydrogenase E2 aa 574-624/647. |
Positive control: | HL-60 cell lysate, Mouse heart tissue lysate, SK-Br-3 cell lysate, K562 cell lysate, Daudi cell lysate, human testis tissue, mouse cerebellum tissue, rat brain tissue. |
Subcellular location: | Mitochondrion matrix. |
Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:200 |
Uniprot #: | SwissProt: P10515 Human | Q8BMF4 Mouse | P08461 Rat |
Alternative names: | 70 kDa mitochondrial autoantigen of primary biliary cirrhosis Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex Dihydrolipoamide S Acetyltransferase Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial DLAT DLTA E2 E2 component of pyruvate dehydrogenase complex M2 antigen complex 70 kDa subunit mitochondrial ODP2_HUMAN PBC PDC E2 PDC-E2 PDCE2 Pyruvate dehydrogenase complex component E2 Pyruvate dehydrogenase complex E2 subunit |
Fig1:
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution. Lane 1: HL-60 cell lysate (20 µg/Lane) Lane 2: Mouse heart tissue lysate (40 µg/Lane) Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: K562 cell lysate Lane 3: Daudi cell lysate Lane 4: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Fig3:
Western blot analysis of Pyruvate Dehydrogenase E2 on different lysates with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Pyruvate Dehydrogenase E2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Pyruvate Dehydrogenase E2 antibody (HA721267) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721267) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |