CTLA-4 Recombinant Rabbit Monoclonal Antibody [PS01-34]
cat.: HA721269
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PS01-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CTLA4 aa 150 to the C-terminus. |
| Positive control: | Human tonsils tissue, human lymph nodes tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IHC-P mIHC |
1:200-1:1,000 1:500 |
| Uniprot #: | SwissProt: P16410 Human | P09793 Mouse |
| Alternative names: | ALPS5 CD 152 CD CD152 CD152 antigen CD152 isoform Celiac disease 3 CELIAC3 CTLA 4 CTLA-4 CTLA4 CTLA4_HUMAN Cytotoxic T cell associated 4 Cytotoxic T lymphocyte antigen 4 Cytotoxic T lymphocyte associated 4 Cytotoxic T lymphocyte associated 4, soluble isoform, included Cytotoxic T lymphocyte associated antigen 4 Cytotoxic T lymphocyte associated antigen 4 short spliced form Cytotoxic T lymphocyte associated protein 4 Cytotoxic T lymphocyte associated serine esterase 4 Cytotoxic T lymphocyte protein 4 Cytotoxic T-lymphocyte protein 4 Cytotoxic T-lymphocyte-associated antigen 4 GRD4 GSE ICOS IDDM12 insulin-dependent diabetes mellitus 12 Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 OTTHUMP00000216623 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Rabbit anti-CTLA-4 antibody (HA721269) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721269) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CTLA-4 antibody (HA721269) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721269) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CTLA-4 antibody (HA721269) at 1/100 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.