RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-15]
cat.: HA721273
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RbBP5 aa 431-480 / 538. |
| Positive control: | SW480 cell lysate, A431 cell lysate, C2C12 cell lysate, 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, K562 cell lysate, N2A cell lysate, PC-12 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q15291 Human | Q8BX09 Mouse Entrez Gene: 304794 Rat |
| Alternative names: | RBBP 5 RBBP-5 RBBP5 RBBP5_HUMAN RBQ 3 RBQ3 Retinoblastoma binding protein 5 Retinoblastoma binding protein RBQ3 Retinoblastoma binding protein5 Retinoblastoma-binding protein 5 Retinoblastoma-binding protein RBQ-3 SWD1 SWD1, Set1c WD40 repeat protein, homolog |
Images
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Fig1:
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lane 3: C2C12 cell lysate Lane 4: 293 cell lysate Lane 5: MCF-7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: K562 cell lysate Lane 8: N2A cell lysate Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721273) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (HA721273) at 1/1,000 dilution. Lane 1: NIH-3T3-si NT cell lysate Lane 2: NIH-3T3-si RbBP5 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. HA721273 was shown to specifically react with RbBP5 in NIH-3T3-si NT cells. Weakened band was observed when NIH-3T3-si RbBP5 sample was tested. NIH-3T3-si NT and NIH-3T3-si RbBP5 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721273, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.