RbBP5 Recombinant Rabbit Monoclonal Antibody [PSH0-16]
cat.: HA721274
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RbBP5 aa 431-480 / 538. |
| Positive control: | Human colon tissue, human colon carcinoma tissue, human mouse tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P |
1:200-1:1,000 |
| Uniprot #: | SwissProt: Q15291 Human | Q8BX09 Mouse |
| Alternative names: | RBBP 5 RBBP-5 RBBP5 RBBP5_HUMAN RBQ 3 RBQ3 Retinoblastoma binding protein 5 Retinoblastoma binding protein RBQ3 Retinoblastoma binding protein5 Retinoblastoma-binding protein 5 Retinoblastoma-binding protein RBQ-3 SWD1 SWD1, Set1c WD40 repeat protein, homolog |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RbBP5 antibody (HA721274) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721274) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RbBP5 antibody (HA721274) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721274) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human mouse tissue with Rabbit anti-RbBP5 antibody (HA721274) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721274) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.