Phospho-RPS6 (S235 + S236) Recombinant Rabbit Monoclonal Antibody [PS01-04]
cat.: HA721275
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PS01-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPS6 aa 200 to the C-terminus (phospho S235 + S236). |
| Positive control: | NIH/3T3 cell lysate, THP-1 cell lysate, Hela cell lysate, rat spleen tissue, Hela. |
| Subcellular location: | Cytosol, Endoplasmic reticulum, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:50 |
| Uniprot #: | SwissProt: P62753 Human | P62754 Mouse | P62755 Rat |
| Alternative names: | 40S ribosomal protein S6 Air8 NP33 Phosphoprotein NP33 Pp30 Ribosomal protein S6 RP S6 rps6 RS6 RS6_HUMAN S6 S6 Ribosomal Protein |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody (HA721275) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721275) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling Phospho-RPS6 (S235 + S236) with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody (HA721275) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody (HA721275) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Western blot analysis of Phospho-RPS6 (S235 + S236) on different lysates with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody (HA721275) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2 : HeLa treated with 100mM Calyculin A for 30min cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721275, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28.7 kDa Observed band size: 33 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.