Phospho-eIF4G (S1108) Recombinant Rabbit Monoclonal Antibody [PS01-08]
cat.: HA721276
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PS01-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 175 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human eIF4G1 (phospho S1108). |
| Positive control: | A431 cell lysate, SK-Br-3 cell lysate, MCF-7 cell lysate, A431. |
| Subcellular location: | Cytosol. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: Q04637 Human |
| Alternative names: | DKFZp686A1451 eIF 4 gamma 1 eIF 4G 1 eIF 4G1 eIF-4-gamma 1 eIF-4G 1 eIF-4G1 EIF4 gamma EIF4F EIF4G EIF4G1 EIF4GI Eukaryotic translation initiation factor 4 gamma 1 IF4G1_HUMAN p220 |
Images
|
Fig1:
Western blot analysis of Phospho-eIF4G (S1108) on different lysates with Rabbit anti-Phospho-eIF4G (S1108) antibody (HA721276) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 175 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721276) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of A431 cells labeling Phospho-eIF4G (S1108) with Rabbit anti-Phospho-eIF4G (S1108) antibody (HA721276) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-eIF4G (S1108) antibody (HA721276) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.