Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17]
cat.: HA721277
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: PSH0-17
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 148 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human AOX1 aa 1,301-1,338 / 1,338.
Positive control: Rat liver tissue lysates, rat liver tissue, rat adrenal gland tissue, human kidney tissue, mouse testis tissue, Hela.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:200-1:1,000
1:500-1:1,000
Uniprot #: SwissProt: Q06278 Human | O54754 Mouse | Q9Z0U5 Rat
Alternative names: Aldehyde oxidase 1 AO AOH1 AOX 1 azaheterocycle hydroxylase EC 1.2.3.1
Images
HA721277_1.jpg Fig1: Western blot analysis of Aldehyde Oxidase on rat liver tissue lysates with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 148 kDa
Observed band size: 148 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721277) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721277_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721277_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721277_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721277_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721277_6.jpg Fig6: Flow cytometric analysis of Hela cells labeling Aldehyde Oxidase.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721277, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.