Aldehyde Oxidase Recombinant Rabbit Monoclonal Antibody [PSH0-17]
cat.: HA721277
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-17 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 148 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AOX1 aa 1,301-1,338 / 1,338. |
| Positive control: | Rat liver tissue lysates, rat liver tissue, rat adrenal gland tissue, human kidney tissue, mouse testis tissue, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:200-1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q06278 Human | O54754 Mouse | Q9Z0U5 Rat |
| Alternative names: | Aldehyde oxidase 1 AO AOH1 AOX 1 azaheterocycle hydroxylase EC 1.2.3.1 |
Images
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Fig1:
Western blot analysis of Aldehyde Oxidase on rat liver lysate with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 41 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721277, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 148 kDa Observed band size: 148 kDa |
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Fig2:
Western blot analysis of Aldehyde Oxidase on mouse liver lysate with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 41 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721277, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 148 kDa Observed band size: 148 kDa |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Aldehyde Oxidase antibody (HA721277) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721277) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Flow cytometric analysis of Hela cells labeling Aldehyde Oxidase. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721277, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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