Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | ELISA(Det) |
Clonality: | Monoclonal |
Clone number: | PS00-82 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4). |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 31 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within full length human IL-1 beta. |
Positive control: | Recombinant IL-1 beta protein |
Subcellular location: | Cytoplasm, Lysosome, Secreted |
Recommended Dilutions:
ELISA (Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS01-39] to IL-1 beta (Capture) (HA721278). |
Uniprot #: | SwissProt: P01584 Human |
Alternative names: | Catabolin H1 IFN beta inducing factor IL 1 IL 1 beta IL-1 beta IL1 IL1 BETA IL1B IL1B_HUMAN IL1F2 Interleukin 1 beta Interleukin 1 beta precursor interleukin 1, beta Interleukin-1 beta OAF Osteoclast activating factor OTTHUMP00000162031 Preinterleukin 1 beta Preinterleukin beta Pro interleukin 1 beta |
Fig1:
The binding activity of IL-1 beta (HA721279) with recombinant IL-1 beta protein. Immobilized recombinant IL-1 beta protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721279) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Standard curve of IL-1 beta matched pair antibodies: Sandwich ELISA analysis of IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721278 [PS01-39] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IL-1 beta protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-82] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |