GM130 Recombinant Rabbit Monoclonal Antibody [JE42-53]
cat.: HA721282
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE42-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 113 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GM130 aa 1-100 (N terminal). |
| Positive control: | MCF-7 cell lysates, mouse kidney tissue, rat kidney tissue, MCF7. |
| Subcellular location: | Cytoplasm, Cytoskeleton, Membrane, Microtubule. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q08379 Human | Q921M4 Mouse | Q62839 Rat |
| Alternative names: | 30 kDa cis Golgi matrix protein 130 kDa cis-Golgi matrix protein Cis golgi matrix protein GM130 GM130 Gm130 autoantigen GOGA2_HUMAN GOLGA 2 Golga2 Golgi autoantigen Golgi autoantigen golgin subfamily a 2 Golgi matrix protein GM130 Golgin 95 golgin A2 Golgin subfamily a 2 Golgin subfamily A member 2 Golgin-95 MGC20672 SY11 protein |
Images
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Fig1:
Western blot analysis of GM130 on MCF-7 cell lysates with Rabbit anti-GM130 antibody (HA721282) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721282) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GM130 antibody (HA721282) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721282) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GM130 antibody (HA721282) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721282) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MCF7 cells labeling GM130 with Rabbit anti-GM130 antibody (HA721282) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GM130 antibody (HA721282) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.