Histone H2A (acetyl K9) Recombinant Rabbit Monoclonal Antibody [PS01-45]
cat.: HA721284
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, CUT&Tag-seq, ChIP |
| Clonality: | Monoclonal |
| Clone number: | PS01-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 14 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Histone H2A aa 1-50 (acetyl K9). |
| Positive control: | HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate, human colon carcinoma tissue, mouse skin tissue, rat skin tissue, NIH/3T3. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell ChIP |
1:1,000 1:4,000 1:50 Use 0.5~2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: P04908 Human | Q93077 Human | Q99878 Human | C0HKE6 Mouse | C0HKE1 Mouse | P02262 Rat |
| Alternative names: | H2A H2A1B_HUMAN H2AFM HIST1H2A HIST1H2AE Histone H2A type 1-B/E Histone H2A.2 Histone H2A/a Histone H2A/m |
Images
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Fig1:
Western blot analysis of Histone H2A (acetyl K9) on different lysates with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate (20 µg/Lane) Lane 2: HeLa treated with 500 ng/mL TSA for 4 hours whole cell lysate (20 µg/Lane) Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721284) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721284) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721284) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721284) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H2A (acetyl K9) with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2A (acetyl K9) antibody (HA721284) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2A (acetyl K9) (HA721284) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
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