Histone H2B (acetyl K20) Recombinant Rabbit Monoclonal Antibody [PS01-46]
cat.: HA721285
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, ChIP
Clonality: Monoclonal
Clone number: PS01-46
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 14 kDa
Isotype: IgG
Immunogen: Synthetic peptide.
Positive control: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate, human colon carcinoma tissue, human skin tissue, mouse skin tissue, rat skin tissue, Hela, NIH/3T3.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  ChIP
1:1,000
1:1,000
1:50
Use 0.5~2 μg for 25 μg of chromatin.
Uniprot #: SwissProt: O60814 Human | P06899 Human | P23527 Human | P33778 Human | P57053 Human | P58876 Human | P62807 Human | Q16778 Human | Q5QNW6 Human | Q8N257 Human | Q93079 Human | Q99877 Human | Q99879 Human | Q99880 Human | P10854 Mouse | Q64475 Mouse | Q00729 Rat
Alternative names: GL105 H2B GL105 H2B histone family member O H2B histone family member S H2B.1 H2B.1 B H2B.b H2B.c H2B.d H2B.e H2B.f H2B.j H2B.q H2B/b H2B/c H2B/d H2B/e H2B/f H2B/j H2B/o H2B/q H2BFB H2BFC H2BFD H2BFE H2BFF H2BFJ H2BFO H2BFQ H2BFS H2BGL105 H2BQ HIRIP2 HIST1H2BB HIST1H2BD HIST1H2BH HIST1H2BL HIST1H2BM HIST1H2BN HIST2H2BE histone cluster 2 Histone H2B histone H2B GL105 Histone H2B type 1 B Histone H2B type 1 D Histone H2B type 1 H Histone H2B type 1 L Histone H2B type 1 M Histone H2B type 1 N Histone H2B type 2 E Histone H2B.q histone protein
Images
HA721285_1.jpg Fig1: Western blot analysis of Histone H2B (acetyl K20) on different lysates with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/1,000 dilution.

Lane 1: Hela whole cell lysate (20 µg/Lane)
Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate (20 µg/Lane)

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 2 minutes;
15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
HA721285_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721285_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721285_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721285_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721285_6.jpg Fig6: Immunocytochemistry analysis of HeLa cells labeling Histone H2B (acetyl K20) with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721285_7.jpg Fig7: Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H2B (acetyl K20) with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2B (acetyl K20) antibody (HA721285) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
HA721285_8.jpg Fig8: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H2B (acetyl K20) (HA721285) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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