Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | ELISA(Cap) |
Clonality: | Monoclonal |
Clone number: | PS00-80 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4). |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 22 kDa |
Isotype: | IgG |
Immunogen: | Recombinant full length protein. |
Positive control: | Recombinant human IFN-alpha protein. |
Subcellular location: | Secreted. |
Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS00-79] to IFN-alpha (Detector) (HA721291). |
Uniprot #: | SwissProt: P01562 Human |
Alternative names: | IFL IFN alpha IFN alpha 1/13 IFN alpha 1b IFN alphaD IFN IFN-alpha-1/13 IFNA@ IFNA1 IFNA1_HUMAN IFNA13 Interferon alpha 1 Interferon alpha-1/13 Interferon alpha-D Interferon alpha1 Interferon, alpha 13 LeIF D |
Fig1:
The binding activity of IFN-alpha (HA721290) with recombinant human IFN-alpha protein. Immobilized recombinant human IFN-alpha protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721290) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Standard curve of IFN-alpha matched pair antibodies: Sandwich ELISA analysis of IFN-alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721290 [PS00-80] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IFN-alpha protein starting from 20 ug/ml to 19 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-79] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |