Human IFN-alpha Recombinant Rabbit Monoclonal Antibody [PS00-80]
cat.: HA721290
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: ELISA(Cap)
Clonality: Monoclonal
Clone number: PS00-80
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Recombinant full length protein.
Positive control: Recombinant human IFN-alpha protein.
Subcellular location: Secreted.
Recommended Dilutions:
  ELISA(Cap)

Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PS00-79] to IFN-alpha (Detector) (HA721291).
Uniprot #: SwissProt: P01562 Human
Alternative names: IFL IFN alpha IFN alpha 1/13 IFN alpha 1b IFN alphaD IFN IFN-alpha-1/13 IFNA@ IFNA1 IFNA1_HUMAN IFNA13 Interferon alpha 1 Interferon alpha-1/13 Interferon alpha-D Interferon alpha1 Interferon, alpha 13 LeIF D
Images
HA721290_1.jpg Fig1: The binding activity of IFN-alpha (HA721290) with recombinant human IFN-alpha protein.

Immobilized recombinant human IFN-alpha protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃, and incubated with the primary antibody (HA721290) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
HA721290_2.jpg Fig2: Standard curve of IFN-alpha matched pair antibodies:

Sandwich ELISA analysis of IFN-alpha matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721290 [PS00-80] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant IFN-alpha protein starting from 20 ug/ml to 19 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-79] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.