PPP3CB Recombinant Rabbit Monoclonal Antibody [PSH0-21]
cat.: HA721292
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, ICC, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform 265-318/524 |
| Positive control: | Mouse brain tissue lysate, Jurkat cell lysate, rat brain tissue lysate, rat skeletal muscle tissue, mouse skeletal muscle tissue, MCF-7. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P ICC FC |
1:1,000 1:200-1:1,000 1:200 1:1000 |
| Uniprot #: | SwissProt: P16298 Human | P48453 Mouse | P20651 Rat |
| Alternative names: | Calcineurin A beta Calcineurin A2 Calcineurin B, formerly Calmodulin dependent calcineurin A subunit beta isoform Calmodulin-dependent calcineurin A subunit beta isoform CALNA 2 CALNA2 CALNB CAM PRP catalytic subunit CAM-PRP catalytic subunit CNA2 CnAbeta PP2BB_HUMAN PP2Bbeta PPP3CB Protein phosphatase 2B, catalytic subunit, beta isoform, formerly Protein phosphatase 3 (formerly 2B) catalytic subunit beta isoform Protein phosphatase 3 catalytic subunit beta isoform Protein phosphatase 3 catalytic subunit beta isozyme Serine/threonine protein phosphatase 2B catalytic subunit beta isoform Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform |
Images
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Fig1:
Western blot analysis of PPP3CB on different lysates with Rabbit anti-PPP3CB antibody (HA721292) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Jurkat cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (HA721292) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-PPP3CB antibody (HA721292) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721292) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-PPP3CB antibody (HA721292) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721292) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of MCF-7 cells labeling PPP3CB with Rabbit anti-PPP3CB antibody (HA721292) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PPP3CB antibody (HA721292) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of MCF-7 cells labeling PPP3CB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721292, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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