Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE35-45 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 51 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human MEF2C aa 400-473. |
Subcellular location: | Cytoplasm, Nucleus |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q06413 Human |
Alternative names: | C5DELq14.3 DEL5q14.3 MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) MADS box transcription enhancer factor 2, polypeptide C MEF2C MEF2C_HUMAN Myocyte enhancer factor 2C Myocyte specific enhancer factor 2C Myocyte-specific enhancer factor 2C OTTHUMP00000222409 Similar to MADS box transcription enhancer factor 2 polypeptide C |
Fig1:
Western blot analysis of MEF2C on different lysates with Rabbit anti-MEF2C antibody (HA721298) at 1/500 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721298) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |