MEF2C Recombinant Rabbit Monoclonal Antibody [JE35-45]
cat.: HA721298
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE35-45
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MEF2C aa 400-473.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q06413 Human
Alternative names: C5DELq14.3 DEL5q14.3 MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) MADS box transcription enhancer factor 2, polypeptide C MEF2C MEF2C_HUMAN Myocyte enhancer factor 2C Myocyte specific enhancer factor 2C Myocyte-specific enhancer factor 2C OTTHUMP00000222409 Similar to MADS box transcription enhancer factor 2 polypeptide C
Images
HA721298_1.jpg Fig1: Western blot analysis of MEF2C on different lysates with Rabbit anti-MEF2C antibody (HA721298) at 1/500 dilution.

Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate
Lane 3: Jurkat cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 55 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721298) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.