B7-H2 Recombinant Rabbit Monoclonal Antibody [JE52-85]
cat.: HA721299
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE52-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 33 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human B7-H2 aa 250-302.
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
1:1,000
Uniprot #: SwissProt: O75144 Human
Alternative names: B7 H2 B7 homolog 2 B7 homologue 2 B7 like protein Gl50 B7 related protein 1 B7-H2 B7-like protein Gl50 B7-related protein 1 B7H2 B7RP 1 B7RP-1 B7RP1 CD 275 CD275 CD275 antigen GL 50 GL50 ICOS L ICOS LG ICOS ligand ICOSL ICOSL_HUMAN Icoslg Inducible T cell co stimulator ligand KIAA0653 LICOS Transmembrane protein B7 H2 ICOS ligand
Images
HA721299_1.jpg Fig1: Western blot analysis of B7-H2 on different lysates with Rabbit anti-B7-H2 antibody (HA721299) at 1/500 dilution.

Lane 1: HUVEC cell lysate, untreated with TNF-alpha for 24h (20ng/mL)
Lane 2: HUVEC cell lysate, treated with TNF-alpha for 24h (20ng/mL)
Lane 3: Daudi cell lysate
Lane 4: Raji cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 33 kDa
Observed band size: 65 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721299) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.