RIPK2 Recombinant Rabbit Monoclonal Antibody [JE51-90]
cat.: HA721300
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE51-90
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RIPK2 aa 500-540.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O43353 Human
Alternative names: CARD 3 CARD carrying kinase CARD containing ICE associated kinase CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD-containing IL-1 beta ICE-kinase CARD-containing interleukin-1 beta-converting enzyme-associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 Receptor-interacting protein 2 Receptor-interacting serine/threonine-protein kinase 2 RICK RIP 2 RIP like interacting CLARP kinase RIP-2 RIP-like-interacting CLARP kinase RIPK 2 Ripk2 RIPK2_HUMAN TNFRSF Tyrosine-protein kinase RIPK......
Images
HA721300_1.jpg Fig1: Western blot analysis of RIPK2 on different lysates with Rabbit anti-RIPK2 antibody (HA721300) at 1/500 dilution.

Lane 1: PC-3M cell lysate
Lane 2: 22RV1 cell lysate
Lane 3: RWPE-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 61.2 kDa
Observed band size: 61 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721300) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.