RIPK2 Recombinant Rabbit Monoclonal Antibody [JE51-90]
cat.: HA721300
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE51-90 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RIPK2 aa 500-540. |
| Positive control: | PC-3M cell lysate, 22Rv1cell lysate, RWPE-1 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O43353 Human |
| Alternative names: | CARD 3 CARD carrying kinase CARD containing ICE associated kinase CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD-containing IL-1 beta ICE-kinase CARD-containing interleukin-1 beta-converting enzyme-associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 Receptor-interacting protein 2 Receptor-interacting serine/threonine-protein kinase 2 RICK RIP 2 RIP like interacting CLARP kinase RIP-2 RIP-like-interacting CLARP kinase RIPK 2 Ripk2 RIPK2_HUMAN TNFRSF Tyrosine-protein kinase RIPK...... |
Images
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Fig1:
Western blot analysis of RIPK2 on different lysates with Rabbit anti-RIPK2 antibody (HA721300) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: 22Rv1cell lysate Lane 3: RWPE-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 2 minutes 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721300) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RIPK2 on different lysates with Rabbit anti-RIPK2 antibody (HA721300) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si RIPK2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721300) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.