PBX1 Recombinant Rabbit Monoclonal Antibody [PSH0-24]
cat.: HA721303
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-24 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PBX1 aa 1-250 / 430. |
| Positive control: | Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, Jurkat cell lysate, A431 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate, rat pancreas tissue lysate, human pancreas tissue, mouse pancreas tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P40424 Human | P41778 Mouse Entrez Gene: 304947 Rat |
| Alternative names: | DKFZp686B09108 Homeo box protein PBX1 Homeo box protein PRL Homeobox protein PBX 1 Homeobox protein PBX1 Homeobox protein PRL MGC126627 PBX 1 Pbx1 PBX1_HUMAN Pre B cell leukemia homeobox 1 Pre B cell leukemia transcription factor 1 Pre-B-cell leukemia transcription factor 1 PRL |
Images
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Fig1:
Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (HA721303) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: MCF-7 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: Mouse testis tissue lysate (40 µg/Lane) Lane 8: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721303) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PBX1 antibody (HA721303) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-PBX1 antibody (HA721303) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721303) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.