Cdc6 Recombinant Rabbit Monoclonal Antibody [JE59-52]
cat.: HA721304
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE59-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser54 of Human Cdc6 aa 41-90 / 560. |
| Positive control: | Hela cell lysate, HepG2 cell lysate, human striated muscle tissue, human testis muscle tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:200 |
| Uniprot #: | SwissProt: Q99741 Human |
| Alternative names: | Cdc 18L Cdc 6 CDC18 (cell division cycle 18, S.pombe, homolog) like CDC18 (S.pombe) Cdc18 related protein CDC18(S.pombe) Cdc18-related protein Cdc18L cdc6 CDC6 cell division cycle 6 homolog CDC6 related protein CDC6-related protein CDC6_HUMAN Cdc6p CELL CYCLE CONTROLLER CDC6 Cell division control protein 6 Cell division control protein 6 homolog Cell division cycle 6 homolog (S. cerevisiae) Cell division cycle 6 homolog Cell division cycle 6, S. cerevisiae, homolog of HsCDC 6 HsCDC18 HsCDC6 p62 p62(cdc 6) p62(cdc6) |
Images
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Fig1:
Western blot analysis of Cdc6 on different lysates with Rabbit anti-Cdc6 antibody (HA721304) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721304) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Cdc6 antibody (HA721304) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis muscle tissue with Rabbit anti-Cdc6 antibody (HA721304) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721304) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.