PSMB2 Recombinant Rabbit Monoclonal Antibody [PSH0-28]
cat.: HA721308
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PSMB2 aa 175-201/201. |
| Positive control: | Jurkat cell lysate, HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, SK-OV-3 cell lysate, HT-29 cell lysate, PC-3M cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P49721 Human |
| Alternative names: | HC7 I Macropain subunit C7 I Macropain subunit C7-I Multicatalytic endopeptidase complex subunit C7 1 Multicatalytic endopeptidase complex subunit C7 I Multicatalytic endopeptidase complex subunit C7-I Proteasome (prosome, macropain) subunit beta type 2 Proteasome beta 2 subunit Proteasome component C7 I Proteasome component C7-I Proteasome subunit beta type-2 PSB2_HUMAN Psmb2 |
Images
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Fig1:
Western blot analysis of PSMB2 on different lysates with Rabbit anti-PSMB2 antibody (HA721308) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: SK-OV-3 cell lysate Lane 6: HT-29 cell lysate Lane 7: PC-3M cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM for 1 hour at room temperature. The primary antibody (HA721308) at 1/1,000 dilution was used in 5% NFDM at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.