MAVS Recombinant Rabbit Monoclonal Antibody [PSH0-30]
cat.: HA721310
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH0-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAVS aa 2-50. |
| Positive control: | MCF7 cell lysate, C2C12 cell lysate, human colon tissue, human endometrium tissue, A431 cell. |
| Subcellular location: | Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome |
| Recommended Dilutions:
WB IHC-P IF-Cell IP |
1:1,000 1:200-1:1,000 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q7Z434 Human |
| Alternative names: | CARD adapter inducing interferon beta CARD adaptor inducing IFN beta Cardif DKFZp666M015 FLJ27482 FLJ41962 IFN B promoter stimulator 1 Interferon beta promoter stimulator protein 1 Ips 1 IPS-1 Ips1 KIAA1271 MAVS MAVS_HUMAN Mitochondrial anti viral signaling protein Mitochondrial Antiviral Signaling Mitochondrial antiviral signaling protein Mitochondrial antiviral-signaling protein Putative NF kappa B activating protein 031N Putative NF-kappa-B-activating protein 031N Virus induced signaling adapter virus induced signaling adaptor Virus-induced-signaling adapter VISA |
Images
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Fig1:
Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (HA721310) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: C2C12 cell lysate Lane 3: A431 cell lysate Lane 4: HeLa cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: THP-1 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 75 kDa The molecular weight observed is consistent with that described in the literature (PMID: 16125763 and 30460894) Exposure time: 10S; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721310) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MAVS antibody (HA721310) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721310) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-MAVS antibody (HA721310) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721310) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX |
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Fig4:
Immunocytochemistry analysis of A431 cells labeling MAVS with Rabbit anti-MAVS antibody (HA721310) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MAVS antibody (HA721310) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
MAVS was immunoprecipitated from 0.2 mg A431 cell lysate with HA721310 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721310 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA721310 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA721310 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 47 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.