GPD2 Recombinant Rabbit Monoclonal Antibody [PSH0-31]
cat.: HA721311
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GPD2 aa 43-727. |
| Positive control: | MCF-7 cell lysate, THP-1 cell lysate, U87-MG cell lysate, HeLa cell lysate, Hela cell. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P43304 Human |
| Alternative names: | GDH2 Glycerol 3 phosphate dehydrogenase 2 (mitochondrial) Glycerol 3 phosphate dehydrogenase 2 Glycerol 3 phosphate dehydrogenase mitochondrial Glycerol-3-phosphate dehydrogenase GPD 2 GPD M GPD-M gpd2 GPDH M GPDH-M GPDM GPDM_HUMAN mGPDH mitochondrial Mitochondrial glycerophosphate dehydrogenase mtGPD OTTHUMP00000162788 OTTHUMP00000204452 OTTHUMP00000204453 OTTHUMP00000204458 |
Images
|
Fig1:
Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (HA721311) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721311) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of Hela cells labeling GPD2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721311, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.