Connexin 43 Recombinant Rabbit Monoclonal Antibody [PSH0-32]
cat.: HA721312
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IHC-Fr
Clonality: Monoclonal
Clone number: PSH0-32
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Connexin 43 aa 219-382.
Positive control: A549 cell lysate, PC-12 cell lysate, mouse heart lysate, human heart tissue, human testis tissue.
Subcellular location: Plasma membrane. Endoplasmic reticulum. Cell junction, gap junction.
Recommended Dilutions:
  WB
  IHC-P
  IHC-Fr

1:1,000
1:1,000-1:10,000
1:500
Uniprot #: SwissProt: P17302 Human | P23242 Mouse | P08050 Rat
Alternative names: Connexin 43 Connexin-43 Cx 43 Cx43 CXA1_HUMAN DFNB38 Gap junction 43 kDa heart protein Gap junction alpha-1 protein Gap junction protein alpha 1 43kDa (connexin 43) Gap junction protein alpha 1 43kDa Gap junction protein alpha like GJA 1 Gja1 GJAL ODD ODDD ODOD SDTY3
Images
HA721312_1.jpg Fig1: Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Connexin 43 antibody (HA721312) at 1/500 dilution.

Important Notice: Antigen retrieval is not required before IHC-Fr staining.

The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721312, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721312_2.jpg Fig2: Immunofluorescence analysis of frozen rat cerebellum tissue with Rabbit anti-Connexin 43 antibody (HA721312) at 1/500 dilution.

Important Notice: Antigen retrieval is not required before IHC-Fr staining.

The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721312, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
HA721312_3.png Fig3: Western blot analysis of Connexin 43 on different lysates with Rabbit anti-Connexin 43 antibody (HA721312) at 1/1,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: PC-12 cell lysate (10 µg/Lane)
Lane 3: Mouse heart lysate (20 µg/Lane)

Predicted band size: 43 kDa
Observed band size: 39-43 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721312) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
HA721312_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit Connexin 43 antibody (HA721312) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit Connexin 43 antibody (HA721312) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit Connexin 43 antibody (HA721312) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mosue cerebellum tissue with Rabbit Connexin 43 antibody (HA721312) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit Connexin 43 antibody (HA721312) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit Connexin 43 antibody (HA721312) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit Connexin 43 antibody (HA721312) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit Connexin 43 antibody (HA721312) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721312_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit Connexin 43 antibody (HA721312) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721312) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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