Menin Recombinant Rabbit Monoclonal Antibody [JE30-97]
cat.: HA721313
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE30-97 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Menin aa 566-615 / 615. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, Rat testis tissue lysate, human lung cancer tissue, human testis tissue. |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200 |
| Uniprot #: | SwissProt: O00255 Human |
| Alternative names: | MEA 1 MEA1 MEN 1 Men1 MEN1_HUMAN Menin Multiple Endocrine Adenomatosis 1 Multiple Endocrine Neoplasia 1 SCG 2 SCG2 Suppressor Candidate Gene 2 Wermer syndrome ZES Zollinger Ellison Syndrome |
Images
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Fig1:
Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (HA721313) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721313, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68 kDa Observed band size: 68 kDa |
|
Fig2:
Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (HA721313) at 1/500 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Menin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721313) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Menin antibody (HA721313) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721313) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Menin antibody (HA721313) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721313) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.