HA721315

EBP1 Recombinant Rabbit Monoclonal Antibody [JE35-48]

cat.: HA721315

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell
Clonality:	Monoclonal
Clone number:	JE35-48

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 44 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human EBP1 aa 325-374 / 394.
Positive control:	K-562 (Human chronic myelogenous leukemia cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C2C12 (Mouse myoblast) cell lysate, C6 (Rat glioma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, mouse cerebellum tissue, rat brain tissue, 293, NIH/3T3, PC-12.
Subcellular location:	Cytoplasm, Nucleus
Recommended Dilutions: WB IHC-P IF-Cell	1:5,000 1:1,000 1:50
Uniprot #:	SwissProt: Q9UQ80 Human \| P50580 mouse \| Q6AYD3
Alternative names:	38kDa AA672939 Cell cycle protein p38 2G4 homolog Cell cycle protein p38-2G4 homolog ErbB-3 binding protein 1 ErbB3 binding protein 1 ErbB3-binding protein 1 ErbB3-binding protein Ebp1 hG4 1 hG4-1 IRES-specific cellular trans-acting factor 45 kDa MGC81621 MGC94070 Mpp1 p38 2G4 Pa2g4 PA2G4_HUMAN Plfap Proliferation associated 2G4 Proliferation associated 2G4, 38-KD Proliferation-associated 2G4, 38kDa Proliferation-associated 2G4, a Proliferation-associated protein 1 Proliferation-associated protein 2G4 Protein p38-2G4 si:dz150i12.2 wu:fb19b11 wu:ft56d05 zgc:86732

Images

Fig1: Western blot analysis of EBP1 on different lysates with Rabbit anti-EBP1 antibody (HA721315) at 1/5,000 dilution.

Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: Ramos (Human Burkitt's lymphoma cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: C2C12 (Mouse myoblast) cell lysate
Lane 5: C6 (Rat glioma cell) cell lysate
Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 12 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721315, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 44 kDa
Observed band size: 44 kDa

Fig2: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunocytochemistry analysis of 293 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
	Fig5: Immunocytochemistry analysis of NIH/3T3 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Fig6: Immunocytochemistry analysis of PC-12 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.