EBP1 Recombinant Rabbit Monoclonal Antibody [JE35-48]
cat.: HA721315
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE35-48 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human EBP1 aa 325-374 / 394. |
| Positive control: | K562 cell lysate, HEK-293 cell lysate, PC-12 cell lysate, mouse cerebellum tissue, rat brain tissue, 293, NIH/3T3, PC-12. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500-1:1,000 1:1,000 1:50 |
| Uniprot #: | SwissProt: Q9UQ80 Human | P50580 mouse | Q6AYD3 |
| Alternative names: | 38kDa AA672939 Cell cycle protein p38 2G4 homolog Cell cycle protein p38-2G4 homolog ErbB-3 binding protein 1 ErbB3 binding protein 1 ErbB3-binding protein 1 ErbB3-binding protein Ebp1 hG4 1 hG4-1 IRES-specific cellular trans-acting factor 45 kDa MGC81621 MGC94070 Mpp1 p38 2G4 Pa2g4 PA2G4_HUMAN Plfap Proliferation associated 2G4 Proliferation associated 2G4, 38-KD Proliferation-associated 2G4, 38kDa Proliferation-associated 2G4, a Proliferation-associated protein 1 Proliferation-associated protein 2G4 Protein p38-2G4 si:dz150i12.2 wu:fb19b11 wu:ft56d05 zgc:86732 |
Images
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Fig1:
Western blot analysis of EBP1 on different lysates with Rabbit anti-EBP1 antibody (HA721315) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: HEK-293 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721315) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-EBP1 antibody (HA721315) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721315) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of 293 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Immunocytochemistry analysis of NIH/3T3 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig6:
Immunocytochemistry analysis of PC-12 cells labeling EBP1 with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EBP1 antibody (HA721315) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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