SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
cat.: HA721316
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE36-20 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SIRP alpha aa 395-504 (Cytoplasmic). |
| Positive control: | THP-1 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, human lymph node tissue, human spleen tissue. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P78324 Human | P97797 Mouse | P97710 Rat |
| Alternative names: | Signal regulatory protein alpha type 1 Bit Brain Ig like molecule with tyrosine based activation motifs Brain Ig-like molecule with tyrosine-based activation motifs Brain immunoglobulin like molecule with tyrosine based activation motifs CD172 antigen like family member A CD172 antigen-like family member A CD172a CD172a antigen Inhibitory receptor SHPS-1 Macrophage fusion receptor MFR MYD 1 Myd 1 antigen MyD-1 antigen p84 Protein tyrosine phosphatase non receptor type substrate 1 PTPNS1 SHP substrate 1 SHPS-1 SHPS1 SHPS1_HUMAN Signal regulatory protein alpha 2 Signal regulatory protein alpha 3 Signal regulatory protein alpha Signal regulatory protein alpha type 2 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 SIRP Sirp-alpha-1 Sirp-alpha-2 Sirp-alpha-3 SIRPA SIRPalpha SIRPalpha1 SIRPalpha2 SIRPalpha3 Tyrosine phosphatase SHP substrate 1 Tyrosine protein p...... |
Images
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Fig1:
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SIRP alpha KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721316) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of SIRP alpha on THP-1 lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 16 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721316, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 75-100 kDa |
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Fig5:
Western blot analysis of SIRP alpha on Mouse brain lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 29 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721316, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 75-100 kDa |
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Fig6:
Western blot analysis of SIRP alpha on THP-1 lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 29 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721316, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 75-100 kDa |
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Fig7:
Western blot analysis of SIRP alpha on HL-60 lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 29 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721316, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 75-100 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.