SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
cat.: HA721316
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE36-20 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SIRP alpha aa 395-504 (Cytoplasmic). |
| Positive control: | THP-1 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, human lymph node tissue, human spleen tissue. |
| Subcellular location: | Membrane |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P78324 Human | P97797 Mouse | P97710 Rat |
| Alternative names: | Signal regulatory protein alpha type 1 Bit Brain Ig like molecule with tyrosine based activation motifs Brain Ig-like molecule with tyrosine-based activation motifs Brain immunoglobulin like molecule with tyrosine based activation motifs CD172 antigen like family member A CD172 antigen-like family member A CD172a CD172a antigen Inhibitory receptor SHPS-1 Macrophage fusion receptor MFR MYD 1 Myd 1 antigen MyD-1 antigen p84 Protein tyrosine phosphatase non receptor type substrate 1 PTPNS1 SHP substrate 1 SHPS-1 SHPS1 SHPS1_HUMAN Signal regulatory protein alpha 2 Signal regulatory protein alpha 3 Signal regulatory protein alpha Signal regulatory protein alpha type 2 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 SIRP Sirp-alpha-1 Sirp-alpha-2 Sirp-alpha-3 SIRPA SIRPalpha SIRPalpha1 SIRPalpha2 SIRPalpha3 Tyrosine phosphatase SHP substrate 1 Tyrosine protein p...... |
Images
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Fig1:
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/500 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: HL-60 cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 55 kDa Observed band size: 75-100 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721316) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of THP-1 cells labeling SIRP alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721316, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.