NEK2 Recombinant Rabbit Monoclonal Antibody [JE37-74]
cat.: HA721317
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NEK2 aa 1-50 / 445. |
| Positive control: | A549 (Human lung adenocarcinoma cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P51955 Human |
| Alternative names: | HSPK 21 HsPK21 NEK 2 NEK2 NEK2_HUMAN NEK2A Never in mitosis A-related kinase 2 NIMA (never in mitosis gene a) related kinase 2 NimA like protein kinase 1 NIMA related kinase 2 NimA related protein kinase 2 NimA-like protein kinase 1 NimA-related protein kinase 2 NLK 1 NLK1 Serine/threonine-protein kinase Nek2 |
Images
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Fig1:
Western blot analysis of NEK2 on different lysates with Rabbit anti-NEK2 antibody (HA721317) at 1/1,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 30 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721317, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa Observed band size: 45 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.