NEK2 Recombinant Rabbit Monoclonal Antibody [JE37-74]
cat.: HA721317
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE37-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NEK2 aa 1-50 / 445. |
| Positive control: | A549 cell lysate, Hela cell lysate. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P51955 Human |
| Alternative names: | HSPK 21 HsPK21 NEK 2 NEK2 NEK2_HUMAN NEK2A Never in mitosis A-related kinase 2 NIMA (never in mitosis gene a) related kinase 2 NimA like protein kinase 1 NIMA related kinase 2 NimA related protein kinase 2 NimA-like protein kinase 1 NimA-related protein kinase 2 NLK 1 NLK1 Serine/threonine-protein kinase Nek2 |
Images
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Fig1:
Western blot analysis of NEK2 on different lysates with Rabbit anti-NEK2 antibody (HA721317) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721317) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.