ZEB1 Recombinant Rabbit Monoclonal Antibody [JE57-64]
cat.: HA721318
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE57-64 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ZEB1 aa 1-180/1124. |
| Positive control: | Hela cell lysate, HUVEC cell lysate, human lung carcinoma tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200 |
| Uniprot #: | SwissProt: P37275 Human |
| Alternative names: | AREB 6 AREB6 BZP Delta crystallin enhancer binding factor 1 DELTA EF1 FECD6 MGC133261 Negative regulator of IL 2 Negative regulator of IL2 NIL 2 A NIL 2 A zinc finger protein NIL 2A NIL-2-A zinc finger protein NIL2A Posterior polymorphous corneal dystrophy 3 PPCD3 Represses interleukin 2 expression TCF 8 TCF-8 TCF8 Transcription factor 8 (represses interleukin 2 expression) Transcription factor 8 ZEB 1 ZEB ZEB1 ZEB1_HUMAN ZFHEP ZFHX 1A ZFHX1A Zinc finger E box binding homeobox 1 Zinc finger E-box-binding homeobox 1 Zinc finger homeodomain enhancer binding protein |
Images
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Fig1:
Western blot analysis of ZEB1 on different lysates with Rabbit anti-ZEB1 antibody (HA721318) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HUVEC cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 124 kDa Observed band size: 200 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721318) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ZEB1 antibody (HA721318) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721318) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.