SPOP Recombinant Rabbit Monoclonal Antibody [JE36-04]
cat.: HA721319
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SPOP aa 291-340 / 374. |
| Positive control: | K-562 (Human chronic myelogenous leukemia cell) cell lysate, LoVo (Human colon cancer cell) cell lysate. |
| Subcellular location: | Nucleus, Nucleus speckle. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: O43791 Human | Q6ZWS8 Mouse |
| Alternative names: | BTBD32 HIB homolog 1 Roadkill homolog 1 Speckle type POZ protein Speckle-type POZ protein SPOP SPOP_HUMAN TEF 2 TEF2 |
Images
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Fig1:
Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (HA721319) at 1/2,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 2: LoVo (Human colon cancer cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721319, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.