SPOP Recombinant Rabbit Monoclonal Antibody [JE36-04]
cat.: HA721319
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SPOP aa 291-340 / 374. |
| Positive control: | K562 cell lysate, LOVO cell lysate. |
| Subcellular location: | Nucleus, Nucleus speckle. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O43791 Human | Q6ZWS8 Mouse |
| Alternative names: | BTBD32 HIB homolog 1 Roadkill homolog 1 Speckle type POZ protein Speckle-type POZ protein SPOP SPOP_HUMAN TEF 2 TEF2 |
Images
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Fig1:
Western blot analysis of SPOP on different lysates with Rabbit anti-SPOP antibody (HA721319) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: LOVO cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721319) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.