Merlin Recombinant Rabbit Monoclonal Antibody [JE31-02]
cat.: HA721320
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE31-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Merlin aa 546-595 / 595.
Positive control: Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate.
Subcellular location: Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: P35240 Human | P46662 Mouse | Q63648 Rat
Alternative names: ACN BANF Bilateral acoustic neuroma MERL_HUMAN Merlin Moesin ezrin radixin like protein Moesin ezrin radizin like Moesin-ezrin-radixin-like protein Neurofibromatosis 2 Neurofibromatosis type 2 Neurofibromatosis2 Neurofibromin 2 Neurofibromin-2 Neurofibromin2 NF 2 Nf2 SCH Schwannomerlin Schwannomin
Images
HA721320_1.jpg Fig1: Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/500 dilution.

Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721320_2.jpg Fig2: Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/2,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Merlin cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.