Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE31-02 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 70 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Merlin aa 546-595 / 595. |
Positive control: | Hela cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
Subcellular location: | Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus. |
Recommended Dilutions:
WB |
1:500-1:1,000 |
Uniprot #: | SwissProt: P35240 Human | P46662 Mouse | Q63648 Rat |
Alternative names: | ACN BANF Bilateral acoustic neuroma MERL_HUMAN Merlin Moesin ezrin radixin like protein Moesin ezrin radizin like Moesin-ezrin-radixin-like protein Neurofibromatosis 2 Neurofibromatosis type 2 Neurofibromatosis2 Neurofibromin 2 Neurofibromin-2 Neurofibromin2 NF 2 Nf2 SCH Schwannomerlin Schwannomin |
Fig1:
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/500 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721320) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Merlin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721320) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |