CD14 Recombinant Rabbit Monoclonal Antibody [PD01-63]
cat.: HA721321
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PD01-63
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 40 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CD14 aa 300-375.
Positive control: NIH/3T3 cell lysate, SiHa cell lysate, human colon carcinoma tissue, human liver tissue, human tonsil tissue.
Subcellular location: Cell membrane, Secreted, Golgi apparatus, Membrane raft.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200-1:1,000
Uniprot #: SwissProt: P08571 Human | P10810 Mouse
Alternative names: CD 14 CD_antigen=CD14 CD14 CD14 antigen CD14 molecule CD14_HUMAN LPS-R Mo2 Monocyte differentiation antigen CD14 Monocyte differentiation antigen CD14 urinary form Monocyte differentiation antigen CD14, membrane-bound form Myeloid cell specific leucine rich glycoprotein Myeloid cell-specific leucine-rich glycoprotein
Images
HA721321_1.jpg Fig1: Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: SiHa cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 40 kDa
Observed band size: 60 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721321) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721321_2.jpg Fig2: Western blot analysis of CD14 on different lysates with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.

Lane 1: SW480-si NT cell lysate
Lane 2: SW480-si CD14 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 40 kDa
Observed band size: 50-60 kDa

Exposure time: 25 seconds;
ECL: merk

4-20% SDS-PAGE gel.

HA721321 was shown to specifically react with CD14 in SW480-si NT cells. Weakened band was observed when SW480-si CD14 sample was tested. SW480-si NT and SW480-si CD14 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721321, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature
HA721321_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721321_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CD14 antibody (HA721321) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721321_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD14 antibody (HA721321) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721321) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.