BAX Recombinant Rabbit Monoclonal Antibody [PD01-44]
cat.: HA721324
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: PD01-44
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Bax aa 50-150 (internal sequence).
Positive control: Human breast tissue, human cervical carcinoma tissue, human kidney tissue.
Subcellular location: Mitochondrion outer membrane, Cytoplasm.
Recommended Dilutions:
  IHC-P

1:200
Uniprot #: SwissProt: Q07812 Human
Alternative names: Apoptosis regulator BAX BAX Bax-protein BAX_HUMAN BAXA Baxdelta2G9 Baxdelta2G9omega Baxdelta2omega Bcl-2-like protein 4 BCL2 associated X protein BCL2 associated X protein omega BCL2 associated X protein transcript variant delta2 Bcl2-L-4 BCL2L4 membrane isoform alpha
Images
HA721324_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAX antibody (HA721324) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721324_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-BAX antibody (HA721324) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721324_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-BAX antibody (HA721324) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721324) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.