MLH1 Recombinant Rabbit Monoclonal Antibody [PD01-61]
cat.: HA721325
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD01-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human MLH1 aa 700-756. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, A549 cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, human appendix tissue, human colon tissue, human B-cell lymphoblastic lymphoma tissue, human NKT cell lymphoma tissue, mouse testis tissue, mouse thymus tissue, human colon carcinoma tissue, rat small intestine tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P40692 Human | Q9JK91 Mouse | P97679 Rat |
| Alternative names: | COCA 2 COCA2 DNA mismatch repair protein Mlh1 FCC 2 FCC2 hMLH 1 hMLH1 HNPCC 2 HNPCC HNPCC2 MGC5172 MLH 1 MLH1 MLH1_HUMAN MutL homolog 1 (E. coli) MutL homolog 1 MutL homolog 1 colon cancer nonpolyposis type 2 MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) MutL protein homolog 1 MutL, E. coli, homolog of, 1 |
Images
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Fig1:
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA721325) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate (negative) Lane 3: A549 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA721325) at 1/2,000 dilution. Lane 1: C2C12 cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721325) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of MLH1 on different lysates with Rabbit anti-MLH1 antibody (HA721325) at 1/2,000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si MLH1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. HA721325 was shown to specifically react with MLH1 in Hela-si NT cells. Weakened band was observed when Hela-si MLH1 sample was tested. Hela-si NT and Hela-si MLH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (HA721325, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoblastic lymphoma tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig11:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-MLH1 antibody (HA721325) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721325) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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