GNAQ Recombinant Rabbit Monoclonal Antibody [JE65-59]
cat.: HA721328
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GNAQ aa 51-150 / 359. |
| Positive control: | Jurkat (Human T-lymphoblastic cells) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, C6 (Rat glioma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, mouse colon tissue, human breast tissue, human thyroid cancer tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus, Nucleus, Nucleus membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:1,000 |
| Uniprot #: | SwissProt: P50148 Human | P21279 Mouse | P82471 Rat |
| Alternative names: | CMC1 G alpha Q G protein alpha Q G protein subunit alpha q G-ALPHA-q GAQ GNAQ GNAQ_HUMAN guanine nucleotide binding protein (G protein), q polypeptide Guanine nucleotide binding protein alpha q guanine nucleotide binding protein G protein q polypeptide Guanine nucleotide binding protein G q subunit alpha Guanine nucleotide-binding protein alpha-q Guanine nucleotide-binding protein G(q) subunit alpha SWS |
Images
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Fig1:
Western blot analysis of GNAQ on different lysates with Rabbit anti-GNAQ antibody (HA721328) at 1/5,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lane 3: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate Lane 5: C6 (Rat glioma cell) cell lysate Lane 6: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721328, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 42 kDa |
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Fig2:
Western blot analysis of GNAQ on different lysates with Rabbit anti-GNAQ antibody (HA721328) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GNAQ KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721328) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-GNAQ antibody (HA721328) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721328) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-GNAQ antibody (HA721328) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721328) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Rabbit anti-GNAQ antibody (HA721328) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721328) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.