CRALBP Recombinant Rabbit Monoclonal Antibody [JE35-09]
cat.: HA721330
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE35-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CRALBP aa 26-75 / 317. |
| Positive control: | Rat eyeball tissue lysate, mouse eyeball tissue lysate, B16F1. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50 1ug/mL |
| Uniprot #: | SwissProt: Q9Z275 Mouse Entrez Gene: 293049 Rat |
| Alternative names: | Cellular retinaldehyde binding protein 1 Cellular retinaldehyde binding protein Cellular retinaldehyde-binding protein MGC3663 Retinaldehyde binding protein 1 Retinaldehyde-binding protein 1 RLBP 1 RLBP1 RLBP1_HUMAN |
Images
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Fig1:
Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (HA721330) at 1/500 dilution. Lane 1: Rat eyeball tissue lysate Lane 2: Mouse eyeball tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36.5 kDa Observed band size: 36.5 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721330) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of B16F1 cells labeling CRALBP with Rabbit anti-CRALBP antibody (HA721330) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRALBP antibody (HA721330) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of B16F1 cells labeling CRALBP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721330, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.