IDO Recombinant Rabbit Monoclonal Antibody [PD00-62]
cat.: HA721331
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell, WB, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PD00-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide derived from the C-terminus of human IDO-1 protein. |
| Positive control: | Human endometrial carcinoma tissue, human lymph nodes tissue, human placenta tissue, HeLa cells treated with 50 ng/mL IFN-gamma for 16 hours, A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate, human tonsils tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
IHC-P IF-Cell WB mIHC |
1:200 1:100 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P14902 Human |
| Alternative names: | 3-dioxygenase I23O1_HUMAN IDO 1 IDO IDO-1 IDO1 INDO indolamine 2,3 dioxygenase Indole 2 3 dioxygenase indoleamine 2 3 dioxygenase 1 indoleamine 2 3 dioxygenase Indoleamine 2,3-dioxygenase 1 Indoleamine pyrrole 2 3 dioxygenase Indoleamine-pyrrole 2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-IDO antibody (HA721331) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721331) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IDO antibody (HA721331) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721331) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-IDO antibody (HA721331) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721331) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells treated with or without 50 ng/mL IFN-gamma for 16 hours labeling IDO with Rabbit anti-IDO antibody (HA721331) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IDO antibody (HA721331) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Western blot analysis of IDO on different lysates with Rabbit anti-IDO antibody (HA721331) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 treated with 50ng/mL IFN-gamma for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721331) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig6: mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-IDO antibody (HA721331) at 1/1,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.