RAP1A + RAP1B Recombinant Rabbit Monoclonal Antibody [JE35-61]
cat.: HA721335
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE35-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RAP1A aa 93-142 / 184. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, HEK-293 cell lysate, HEK-293. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoplasm, perinuclear region, Cell junction, Early endosome; Cell membrane, Cytoplasm, cytosol, Cell junction. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P62834 Human | P61224 Human | P62835 Mouse | Q99JI6 Mouse |
| Alternative names: | C21KG G 22K G-22K GTP binding protein smg p21A GTP-binding protein smg p21A KREV 1 KREV1 OTTHUMP00000013741 RAP 1A RAP1 RAP1A RAP1A member of RAS oncogene family RAP1A_HUMAN Ras related protein Krev 1 Ras related protein Rap 1A RAS related protein RAP1A Ras-related protein Krev-1 Ras-related protein Rap-1A SMGP21 GTP-binding protein smg p21B K-REV OK/SW-cl.11 RAL1B rap1b RAP1B member of RAS oncogene family RAP1B_HUMAN Ras family small GTP binding protein RAP1B RAS related protein RAP1B Ras-related protein Rap-1b Small GTP binding protein |
Images
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Fig1:
Western blot analysis of RAP1A + RAP1B on different lysates with Rabbit anti-RAP1A + RAP1B antibody (HA721335) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721335) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HEK-293 cells labeling RAP1A + RAP1B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721335, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.