SorLA Recombinant Rabbit Monoclonal Antibody [JE35-52]
cat.: HA721337
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 248 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SorLA aa 1,341-1,540 / 2,214. |
| Positive control: | Jurkat cell lysates, mouse cerebellum tissue, rat cerebellum tissue. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:500 |
| Uniprot #: | SwissProt: Q92673 Human | O88307 Mouse | P0DSP1 Rat |
| Alternative names: | C11orf32 FLJ21930 FLJ39258 gp250 LDLR relative with 11 ligand binding repeats LDLR relative with 11 ligand-binding repeats Low density lipoprotein receptor relative with 11 ligand binding repeats Low-density lipoprotein receptor relative with 11 ligand-binding repeats LR 11 LR11 LRP 9 LRP9 Mosaic protein LR11 SORL 1 SORL_HUMAN SORL1 SorLA 1 SorLA SorLA-1 Sortilin related receptor Sortilin related receptor L(DLR class) A repeats containing Sortilin-related receptor Sorting protein related receptor containing LDLR class A repeats Sorting protein-related receptor containing LDLR class A repeats |
Images
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Fig1:
Western blot analysis of SorLA on different lysates with Rabbit anti-SorLA antibody (HA721337) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-SorLA KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 248 kDa Observed band size: 300 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721337) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SorLA on Jurkat cell lysates with Rabbit anti-SorLA antibody (HA721337) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 248 kDa Observed band size: 300 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721337) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SorLA antibody (HA721337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SorLA antibody (HA721337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.