Cofilin Recombinant Rabbit Monoclonal Antibody [PSH0-38]
cat.: HA721340
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cofilin aa 117-166 / 166. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, MDA-MB-468 cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, Jurkat cell lysate, COS-1 cell lysate, VERO cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Neuro-2a cell lysate, HeLa, NIH/3T3. |
| Subcellular location: | Nucleus matrix, Cytoplasm, Cytoskeleton, Cell projection. |
| Recommended Dilutions:
WB FC |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P23528 Human | P18760 Mouse | P45592 Rat |
| Alternative names: | 18 kDa phosphoprotein CFL 1 CFL CFL1 COF1_HUMAN Cofilin 1 Cofilin 1 non muscle Cofilin Cofilin non muscle isoform Cofilin-1 epididymis secretory protein Li 15 HEL-S-15 non-muscle isoform p18 |
Images
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Fig1:
Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (HA721340) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HEK-293 cell lysate (30 µg/Lane) Lane 3: MCF7 cell lysate (30 µg/Lane) Lane 4: MDA-MB-468 cell lysate (24 µg/Lane) Lane 5: K-562 cell lysate (16 µg/Lane) Lane 6: SH-SY5Y cell lysate (22 µg/Lane) Lane 7: HUVEC cell lysate (30 µg/Lane) Lane 8: Jurkat cell lysate (30 µg/Lane) Lane 9: COS-1 cell lysate (30 µg/Lane) Lane 10: VERO cell lysate (30 µg/Lane) Lane 11: NIH/3T3 cell lysate (22 µg/Lane) Lane 12: PC-12 cell lysate (30 µg/Lane) Lane 13: Neuro-2a cell lysate (19 µg/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721340) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721340, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Flow cytometric analysis of NIH/3T3 cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721340, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.