WNT8A Recombinant Rabbit Monoclonal Antibody [PSH0-39]
cat.: HA721341
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38.8 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WNT8A aa 100-300 / 351. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse embryo tissue lysate, rat brain tissue lysate, NIH/3T3, HeLa. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9H1J5 Human | Q64527 Mouse Entrez Gene: 291678 Rat |
| Alternative names: | Protein Wnt-8a Protein Wnt-8d wingless type MMTV integration site family, member 8A WNT 8A Wnt 8d wnt8a WNT8A_HUMAN WNT8D |
Images
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Fig1:
Western blot analysis of WNT8A on different lysates with Rabbit anti-WNT8A antibody (HA721341) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: SW480 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (30 µg/Lane) Lane 5: Raji cell lysate (30 µg/Lane) Lane 6: NIH/3T3 cell lysate (30 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Lane 8: Mouse embryo tissue lysate (40 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 38.8 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721341) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling WNT8A with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WNT8A antibody (HA721341) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling WNT8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721341, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling WNT8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721341, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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