RTN3 Recombinant Rabbit Monoclonal Antibody [PSH0-40]
cat.: HA721342
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: PSH0-40
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 112.6 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RTN3 aa 1-500 / 1,032.
Positive control: HeLa cell lysate, HEK-293 cell lysate, U87-MG cell lysate, A549 cell lysate, SH-SY5Y cell lysate, PC-12 cell lysate, L6 cell lysate, human cerebellum tissue lysate, PC-12, HeLa.
Subcellular location: Endoplasmic reticulum membrane, Golgi apparatus membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC

1:1,000
1:50
1:500-1:1,000
Uniprot #: SwissProt: O95197 Human | Q6RJR6 Rat
Alternative names: ASYIP Neuroendocrine specific protein like 2 Neuroendocrine-specific protein-like 2 Neuroendocrine-specific protein-like II NSP like protein II NSP-like protein 2 NSP-like protein II NSPL2 NSPLII Reticulon 3 Reticulon-3 Reticulon3 Rtn3 RTN3_HUMAN
Images
HA721342_1.jpg Fig1: Western blot analysis of RTN3 on different lysates with Rabbit anti-RTN3 antibody (HA721342) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (32 µg/Lane)
Lane 2: HEK-293 cell lysate (33 µg/Lane)
Lane 3: U87-MG cell lysate (30 µg/Lane)
Lane 4: A549 cell lysate (26 µg/Lane)
Lane 5: SH-SY5Y cell lysate (18 µg/Lane)
Lane 6: PC-12 cell lysate (23 µg/Lane)
Lane 7: L6 cell lysate (14 µg/Lane)
Lane 8: Human cerebellum tissue lysate (40 µg/Lane)

Lysates/proteins at 10 µg/Lane.

Predicted band size: 112.6 kDa
Observed band size: 100 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721342) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
HA721342_2.jpg Fig2: Immunocytochemistry analysis of PC-12 cells labeling RTN3 with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RTN3 antibody (HA721342) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721342_3.jpg Fig3: Flow cytometric analysis of HeLa cells labeling RTN3.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721342, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.