IRAK4 Recombinant Rabbit Monoclonal Antibody [JE41-77]
cat.: HA721347
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE41-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human IRAK4 aa 1-100 (N terminal). |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, COS-1 cell lysate, HL-60 cell lysate, human skeletal muscle tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:1,000 |
| Uniprot #: | SwissProt: Q9NWZ3 Human | Q8R4K2 Mouse |
| Alternative names: | IL-1 receptor-associated kinase 4 Interleukin 1 receptor associated kinase 4 mutant form 1 Interleukin-1 receptor-associated kinase 4 Interleukin1 receptor associated kinase 4 IPD1 IRAK 4 IRAK-4 IRAK4 IRAK4 mutated form 1 IRAK4_HUMAN LOC 51135 NY REN 64 NY REN 64 antigen NY-REN-64 REN64 Renal carcinoma antigen NY-REN-64 |
Images
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Fig1:
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (HA721347) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 22 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721347) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (HA721347) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721347) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of IRAK4 on different lysates with Rabbit anti-IRAK4 antibody (HA721347) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: HL-60 cell lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721347) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-IRAK4 antibody (HA721347) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721347) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.