Arp2 Recombinant Rabbit Monoclonal Antibody [JE40-62]
cat.: HA721348
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE40-62 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Arp2 aa 345-394 / 394. |
| Positive control: | Jurkat cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, human heart tissue, SH-SY5Y, rat spleen tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cell projection, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:250 |
| Uniprot #: | SwissProt: P61160 Human | P61161 Mouse | Q5M7U6 Rat |
| Alternative names: | Actin like protein 2 Actin related protein 2 Actin-like protein 2 Actin-related protein 2 ACTR2 ARP2 actin related protein 2 homolog (yeast) ARP2 ARP2/3 COMPLEX ARP2_HUMAN OTTHUMP00000159937 OTTHUMP00000202270 |
Images
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Fig1:
Western blot analysis of Arp2 on different lysates with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721348, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 45 kDa Observed band size: 40 kDa |
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Fig2:
Western blot analysis of Arp2 on Jurkat cell lysate with Rabbit anti-Arp2 antibody (HA721348) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721348, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 44.8 kDa Observed band size: 45 kDa |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Arp2 antibody (HA721348) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721348) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling Arp2 with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Arp2 antibody (HA721348) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.