EEF2 Recombinant Rabbit Monoclonal Antibody [JE32-57]
cat.: HA721349
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE32-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human EEF2 aa 809-858 / 858. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat spleen tissue lysate, HeLa, NIH/3T3. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC IF-Cell |
1:1,000-1:2,000 1:500-1:1,000 1:250 |
| Uniprot #: | SwissProt: P13639 Human | P58252 Mouse | P05197 Rat |
| Alternative names: | EEF 2 Eef2 EF-2 EF2 EF2_HUMAN Elongation factor 2 Eukaryotic translation elongation factor 2 Polypeptidyl tRNA translocase SCA26 |
Images
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Fig1:
Western blot analysis of EEF2 on different lysates with Rabbit anti-EEF2 antibody (HA721349) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721349) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa cells labeling EEF2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721349, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Flow cytometric analysis of NIH/3T3 cells labeling EEF2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721349, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling EEF2 with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of NIH/3T3 cells labeling EEF2 with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.