EEF2 Recombinant Rabbit Monoclonal Antibody [JE32-57]
cat.: HA721349
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC, IF-Cell
Clonality: Monoclonal
Clone number: JE32-57
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 95 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human EEF2 aa 809-858 / 858.
Positive control: HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, rat spleen tissue lysate, HeLa, NIH/3T3.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  FC
  IF-Cell

1:1,000-1:2,000
1:500-1:1,000
1:250
Uniprot #: SwissProt: P13639 Human | P58252 Mouse | P05197 Rat
Alternative names: EEF 2 Eef2 EF-2 EF2 EF2_HUMAN Elongation factor 2 Eukaryotic translation elongation factor 2 Polypeptidyl tRNA translocase SCA26
Images
HA721349_1.jpg Fig1: Western blot analysis of EEF2 on different lysates with Rabbit anti-EEF2 antibody (HA721349) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse liver tissue lysate (40 µg/Lane)
Lane 6: Rat spleen tissue lysate (40 µg/Lane)

Predicted band size: 95 kDa
Observed band size: 95 kDa

Exposure time: 8 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721349) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721349_2.jpg Fig2: Flow cytometric analysis of HeLa cells labeling EEF2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721349, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721349_3.jpg Fig3: Flow cytometric analysis of NIH/3T3 cells labeling EEF2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721349, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
HA721349_4.jpg Fig4: Immunocytochemistry analysis of HeLa cells labeling EEF2 with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721349_5.jpg Fig5: Immunocytochemistry analysis of NIH/3T3 cells labeling EEF2 with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-EEF2 antibody (HA721349) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.