KDM4B Recombinant Rabbit Monoclonal Antibody [JE36-58]
cat.: HA721350
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE36-58
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 122 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human KDM4B aa 97-146 / 1,096.
Positive control: HeLa cell lysate, HCT116 cell lysate, SW480 cell lysate, mouse testis tissue lysate, rat testis tissue lysates, human breast carcinoma tissue, HeLa, SW480.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell

1:1,000-1:2,000
1:200
1:250
Uniprot #: SwissProt: O94953 Human | Q91VY5 Mouse
Alternative names: JHDM3B JmjC domain-containing histone demethylation protein 3B JMJD2B Jumonji domain containing 2B Jumonji domain containing protein 2B Jumonji domain-containing protein 2B Kdm4b KDM4B_HUMAN KIAA0876 Lysine (K) specific demethylase 4B Lysine demethylase 4B Lysine-specific demethylase 4B TDRD14B Tudor domain containing 14B
Images
HA721350_1.jpg Fig1: Western blot analysis of KDM4B on different lysates with Rabbit anti-KDM4B antibody (HA721350) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HCT116 cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)

Predicted band size: 121 kDa
Observed band size: 150 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721350_2.jpg Fig2: Western blot analysis of KDM4B on rat testis tissue lysates with Rabbit anti-KDM4B antibody (HA721350) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 122 kDa
Observed band size: 140 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721350) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721350_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-KDM4B antibody (HA721350) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721350) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721350_4.jpg Fig4: Immunocytochemistry analysis of HeLa cells labeling KDM4B with Rabbit anti-KDM4B antibody (HA721350) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM4B antibody (HA721350) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721350_5.jpg Fig5: Immunocytochemistry analysis of SW480 cells labeling KDM4B with Rabbit anti-KDM4B antibody (HA721350) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM4B antibody (HA721350) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.