Poliovirus Receptor Recombinant Rabbit Monoclonal Antibody [JE45-53]
cat.: HA721351
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE45-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | HUVEC cell lysate, U87-MG cell lysate, A549 cell lysates, HUVEC. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000-1:2,000 1:100 |
| Uniprot #: | SwissProt: P15151 Human |
| Alternative names: | CD155 CD155 antigen FLJ25946 HVED mE4 NECL 5 Necl-5 Necl5 Nectin like 5 Nectin like protein 5 Nectin-like protein 5 Ortholog of mouse Tage4 Poliovirus receptor PVR PVR_HUMAN PVS Taa1 Tage 4 TAGE4 |
Images
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Fig1:
Western blot analysis of Poliovirus Receptor on different lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: U87-MG cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 75 kDa Exposure time: 4 minutes 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Poliovirus Receptor on A549 cell lysates with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 75 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721351) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of HUVEC cells labeling Poliovirus Receptor with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Poliovirus Receptor antibody (HA721351) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.