Cathepsin H Recombinant Rabbit Monoclonal Antibody [JE56-25]
cat.: HA721352
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE56-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cathepsin H aa 180-230. |
| Positive control: | PC-3M cell lysates, PANC-1. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50 |
| Uniprot #: | SwissProt: P09668 Human |
| Alternative names: | Pro-cathepsin H Cathepsin H mini chain Cathepsin H EC:3.4.22.16 Cathepsin H heavy chain Cathepsin H light chain CTSH CPSB |
Images
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Fig1:
Western blot analysis of Cathepsin H on different lysates with Rabbit anti-Cathepsin H antibody (HA721352) at 1/500 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Cathepsin H cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 42 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721352) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Cathepsin H on PC-3M cell lysates with Rabbit anti-Cathepsin H antibody (HA721352) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 42 kDa Exposure time: 3 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721352) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of PANC-1 cells labeling Cathepsin H with Rabbit anti-Cathepsin H antibody (HA721352) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin H antibody (HA721352) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.