FADD Recombinant Rabbit Monoclonal Antibody [JE45-25]
cat.: HA721353
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE45-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | A431 cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, SK-Br-3 cell lysate, SW480 cell lysate, THP-1 cell lysate, A549 cell lysate, HepG2 cell lysate, SW480, human stomach tissue. |
| Subcellular location: | Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:50 |
| Uniprot #: | SwissProt: Q13158 Human |
| Alternative names: | FADD FADD protein FADD_HUMAN Fas (TNFRSF6) associated via death domain Fas associated via death domain Fas associating death domain containing protein Fas associating protein Fas associating protein with death domain Fas TNFRSF6 associated via death domain FAS-associated death domain protein FAS-associating death domain-containing protein GIG 3 GIG3 Growth inhibiting gene 3 protein Growth-inhibiting gene 3 protein H sapiens mRNA for mediator of receptor induced toxicity Mediator of receptor induced toxicity MGC8528 MORT 1 MORT1 Protein FADD |
Images
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Fig1:
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA721353) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 µg/Lane Lane 2: K-562 cell lysate, 10 µg/Lane Lane 3: Jurkat cell lysate, 10 µg/Lane Lane 4: MCF7 cell lysate, 10 µg/Lane Lane 5: HeLa cell lysate, 10 µg/Lane Lane 6: SK-Br-3 cell lysate, 10 µg/Lane Lane 7: SW480 cell lysate, 10 µg/Lane Lane 8: THP-1 cell lysate, 10 µg/Lane Lane 9: A549 cell lysate, 10 µg/Lane Lane 10: HepG2 cell lysate, 10 µg/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721353) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA721353) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 10 µg/Lane Lane 2: SW480 cell lysate, 10 µg/Lane Lane 3: Jurkat cell lysate, 10 µg/Lane Lane 4: A431 cell lysate, 10 µg/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721353) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody (HA721353) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721353) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.