Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, FC, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE35-69 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 53 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human P70 S6 Kinase beta aa 1-150 / 482. |
Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, mouse liver tissue lysate, mouse kidney tissue lysate, HeLa, MCF7, human colon tissue. |
Subcellular location: | Cytoplasm, Nucleus. |
Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:1,000 1:100 1:500-1:1,000 1:1,000 |
Uniprot #: | SwissProt: Q9UBS0 Human | Q9Z1M4 Mouse |
Alternative names: | 70 kDa ribosomal protein S6 kinase 2 EC 2.7.11.1 KS6B2_HUMAN p70 beta p70 ribosomal S6 kinase beta p70 S6 kinase beta p70 S6K-beta p70 S6KB p70 S6Kbeta p70(S6K) beta p70-beta p70-S6K 2 P70S6K2 p70S6Kb Ribosomal protein S6 kinase 70kDa, polypeptide 2 Ribosomal protein S6 kinase B2 Ribosomal protein S6 kinase beta 2 Ribosomal protein S6 kinase beta-2 Rps6kb2 S6 kinase related kinase S6 kinase-related kinase S6K beta 2 S6K beta S6K-beta S6K-beta-2 S6K2 Serine/threonine protein kinase 14 beta Serine/threonine-protein kinase 14B SRK STK14B |
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Fig1:
Western blot analysis of P70 S6 Kinase beta on different lysates with Rabbit anti-P70 S6 Kinase beta antibody (HA721354) at 1/1,000 dilution. Lane 1: HeLa cell lysate, 20 µg/Lane Lane 2: Jurkat cell lysate, 20 µg/Lane Lane 3: HEK-293 cell lysate, 20 µg/Lane Lane 4: Mouse liver tissue lysate, 40 µg/Lane Lane 5: Mouse kidney tissue lysate, 40 µg/Lane Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721354) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of P70 S6 Kinase beta on different lysates with Rabbit anti-P70 S6 Kinase beta antibody (HA721354) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si P70 S6 Kinase beta cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling P70 S6 Kinase beta with Rabbit anti-P70 S6 Kinase beta antibody (HA721354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-P70 S6 Kinase beta antibody (HA721354) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of MCF7 cells labeling P70 S6 Kinase beta. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-P70 S6 Kinase beta antibody (HA721354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |