GTPase HRAS Recombinant Rabbit Monoclonal Antibody [JE42-15]
cat.: HA721355
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE42-15 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GTPase HRAS aa 1-50. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat brain tissue lysate, mouse kidney tissue lysate, human stomach carcinoma tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus, Golgi apparatus membrane; Nucleus, Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P01112 Human | Q61411 Mouse | P20171 Rat |
| Alternative names: | C BAS/HAS c H ras C HA RAS1 c has/bas p21 protein c ras Ki 2 activated oncogene c-H-ras CTLO GTP and GDP binding peptide B GTPase HRas, N-terminally processed H Ras 1 H RASIDX H-Ras-1 Ha Ras Ha Ras1 proto oncoprotein Ha-Ras HAMSV Harvey rat sarcoma viral oncogene homolog Harvey rat sarcoma viral oncoprotein HRAS HRAS1 K ras N ras p19 H RasIDX protein p21ras Ras family small GTP binding protein H Ras RASH_HUMAN RASH1 Transformation gene oncogene HAMSV Transforming protein p21 v Ha ras Harvey rat sarcoma viral oncogene homolog VH Ras vHa RAS |
Images
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Fig1:
Western blot analysis of GTPase HRAS on different lysates with Rabbit anti-GTPase HRAS antibody (HA721355) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 µg/Lane) Lane 2: Jurkat cell lysate (40 µg/Lane) Lane 3: NIH/3T3 cell lysate (40 µg/Lane) Lane 4: PC-12 cell lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (10 µg/Lane) Lane 6: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-GTPase HRAS antibody (HA721355) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721355) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.